Genome-Wide Association Analysis Reveals Different Genetic Control in Panicle Architecture Between Indica and Japonica Rice

Panicle architecture determines the number of spikelets per panicle (SPP) and is highly associated with grain yield in rice (Oryza sativa L.). Understanding the genetic basis of panicle architecture is important for improving the yield of rice grain. In this study, we dissected panicle architecture traits into eight components, which were phenotyped from a germplasm collection of 529 cultivars. Multiple regression analysis revealed that the number of secondary branch (NSB) was the major factor that contributed to SPP. Genome-wide association analysis was performed independently for the eight particle architecture traits observed in the indica and japonica rice subpopulations compared with the whole rice population. In total, 30 loci were associated with these traits. Of these, 13 loci were closely linked to known panicle architecture genes, and 17 novel loci were repeatedly identified in different environments. An association signal cluster was identified for NSB and number of spikelets per secondary branch (NSSB) in the region of 31.6 to 31.7 Mb on chromosome 4. In addition to the common associations detected in both indica and japonica subpopulations, many associated loci were unique to one subpopulation. For example, Ghd7 and DST were specifically associated with panicle length (PL) in indica and japonica rice, respectively. Moreover, the Ghd7-mediated flowering genes Hd17 and Ehd1 were associated with the formation of panicle architecture in indica rice. These results suggest that different gene networks regulate panicle architecture in indica and japonica rice. Rice cultivars have been developed for thousands of years in Asia. The long period of domestication of wild rice (Oryza rufipogon Griff.) has involved natural and artificial selection as well as recent rapid genetic improvement. High grain yield is one of the major goals of modern breeders and panicle architecture is one of the most important traits related to grain yield in rice. Panicle architecture is rich in variation including long or short panicles, erect and dense panicles, or sparse panicles. Understanding the genetic basis of panicle architecture is critical to efficiently breed high-yielding varieties. Panicle development initiates during the transition from vegetative growth to reproductive growth. The shoot apical meristem is transformed into the inflorescence Published in Plant Genome Volume 9. doi: 10.3835/plantgenome2015.11.0115 © Crop Science Society of America 5585 Guilford Rd., Madison, WI 53711 USA This is an open access article distributed under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China. Received 11 Nov. 2015. Accepted 22 Feb. 2016. *Corresponding author (yzxing@